In churches which use the Byzantine Rite, the Trisagion is chanted immediately before the Prokeimenon and the Epistle reading. It is also included in a set of prayers named for it, called the Trisagion Prayers, which forms part of numerous services (the Hours, Vespers, Matins, and as part of the opening prayers for most services).
In the Latin Church, the main regular use of the Trisagion is on Good Friday, when it is sung throughout the ceremony of the Adoration of the Cross.
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